A technically impressive study, combining in vivo patch-clamp recording of dendrites together with calcium imging of the soma in primary visual cortex of anaesthetized and awake mice, demonstrates that dendritic spikes occur in vivo and contribute to orientation selectivity.
Two-photon calcium imaging of somatic and dendritic calcium transients, with simultaneous dendritic recordings, provided the most direct evidence of local dendritic spiking.
By zooming into the mouse visual system with a two-photon microscope, Smith and Hausser provide important insights into how cortex produces diverse functional tuning under the constraint of low divergence. Their work will serve as a valuable reference for imaging the visual system at finer resolution in other species.
It's a remarkable discovery — one that's [providing] important insights into the role that dendrites play in brain circuitry and function.
This is a reference point to minimize electrode size and increase the spatial density in MEAs [microelectrode arrays].